anti-mouse il-27p28 mm27-b1 antibody  (Bio X Cell)

Journal: Journal of neuroinflammation

Article Title: The cytokine IL-27 reduces inflammation and protects photoreceptors in a mouse model of retinal degeneration.

doi: 10.1186/s12974-022-02576-x

Figure Lengend Snippet: Fig. 5 IL-27 localization in the retina. a (Upper panels) Immunodetection of IL-27p28 in rd10 mice at age P25. Colocalization of IL-27p28 signal (red) with GFAP-positive processes (green) indicated by asterisks, consistent with expression in Muller glia. Brightness was enhanced in the GFAP panel in order to visualize the processes in the ONL. (Lower panels) Immunodetection of IL-27p28 in primary Muller glia cultures (red). GFAP (green) detection on an adjacent coverslip indicates the purity of the cultures because every cell was GFAP-positive. The “no Ab” panel contains primary Muller glia with no primary antibody at the equivalent exposure settings as the IL-27 image to demonstrate lack of non-specific immunodetection. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer. b DNA gel with PCR products showing IL-27 and IL-27Rα amplification in the BV2 mouse microglia cell line, primary Muller glia (MG) and whole retina from rd10 mice

Article Snippet: The slides were washed in PBS, incubated in blocking buffer (10% goat serum in 0.3% Triton X-100/PBS) for 30 min then incubated overnight at 4 °C in primary antibody solution containing anti-Iba1 antibody (Wako, Richmond, VA, 1:200 dilution), anti-IL-27p28 antibody (Novus, Centennial, CO, 1:100 dilution) or anti-GFAP (Invitrogen, 1:400 dilution), diluted in 2% goat serum in 0.3% Triton X-100/ PBS.

Techniques: Immunodetection, Expressing, Amplification

Journal: Journal of Neuroinflammation

Article Title: The cytokine IL-27 reduces inflammation and protects photoreceptors in a mouse model of retinal degeneration

doi: 10.1186/s12974-022-02576-x

Figure Lengend Snippet: IL-27 localization in the retina. a (Upper panels) Immunodetection of IL-27p28 in rd10 mice at age P25. Colocalization of IL-27p28 signal (red) with GFAP-positive processes (green) indicated by asterisks, consistent with expression in Muller glia. Brightness was enhanced in the GFAP panel in order to visualize the processes in the ONL. (Lower panels) Immunodetection of IL-27p28 in primary Muller glia cultures (red). GFAP (green) detection on an adjacent coverslip indicates the purity of the cultures because every cell was GFAP-positive. The “no Ab” panel contains primary Muller glia with no primary antibody at the equivalent exposure settings as the IL-27 image to demonstrate lack of non-specific immunodetection. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer. b DNA gel with PCR products showing IL-27 and IL-27Rα amplification in the BV2 mouse microglia cell line, primary Muller glia (MG) and whole retina from rd10 mice

Article Snippet: The slides were washed in PBS, incubated in blocking buffer (10% goat serum in 0.3% Triton X-100/PBS) for 30 min then incubated overnight at 4 °C in primary antibody solution containing anti-Iba1 antibody (Wako, Richmond, VA, 1:200 dilution), anti-IL-27p28 antibody (Novus, Centennial, CO, 1:100 dilution) or anti-GFAP (Invitrogen, 1:400 dilution), diluted in 2% goat serum in 0.3% Triton X-100/PBS.

Techniques: Immunodetection, Expressing, Amplification

Journal: Mediators of Inflammation

Article Title: Interleukin-27 Ameliorates Atherosclerosis in ApoE −/− Mice through Regulatory T Cell Augmentation and Dendritic Cell Tolerance

doi: 10.1155/2022/2054879

Figure Lengend Snippet: Recombinant IL-27 inhibited the initiation of atherosclerosis. (a), (b), and (c), Representative photomicrographs show Oil Red O and hematoxylin stained aortic root sections from groups treated with IL-27, anti-IL-27p28-Ab, or PBS while they were fed a high-fat diet for 8 weeks, respectively. (g) Data from group (a), (b), and (c) are shown. A black bar represents 200 μ m. (d), (e), and (f) Representative photomicrographs show Oil Red O stained the descending aortas from groups treated with IL-27, anti-IL-27p28-Ab, or PBS while they were fed a high-fat diet for 8 weeks, respectively. (h) Data from group (d), (e), and (f) are shown. ∗∗∗ P < 0.001, ∗∗ P < 0.01, ∗ P < 0.05.

Article Snippet: Groups A, B, and C received intraperitoneal injections of recombinant IL-27 (BioLegend, San Diego, CA; 500 ng every three days), an anti-IL-27p28 antibody (anti-IL-27p28-Ab; eBioscience, San Diego, CA; 50 μ g every three days), or phosphate-buffered saline (PBS) (0.1 mL every three days) for 8 weeks, while groups D, E, and F started treatment after 8 weeks of Western-type diet consumption and were treated for 8 weeks.

Techniques: Recombinant, Staining

Journal: Mediators of Inflammation

Article Title: Interleukin-27 Ameliorates Atherosclerosis in ApoE −/− Mice through Regulatory T Cell Augmentation and Dendritic Cell Tolerance

doi: 10.1155/2022/2054879

Figure Lengend Snippet: Recombinant IL-27 inhibited the progression of atherosclerosis. (a), (b), and (c), Representative photomicrographs show Oil Red O and hematoxylin stained aortic root sections from groups treated with IL-27, anti-IL-27p28-Ab, or PBS for another 8 weeks after 8 weeks of western diet consumption, respectively. (g) Data from group (a), (b), and (c) are shown. A black bar represents 200 μ m. (d, e, f), representative photomicrographs show Oil Red O stained the descending aortas from groups treated with IL-27, anti-IL-27p28-Ab, or PBS for another 8 weeks after 8 weeks of western diet consumption, respectively. (h) Data from group (d), (e), and (f) are shown. ∗∗∗ P < 0.001, ∗∗ P < 0.01, ∗ P < 0.05.

Article Snippet: Groups A, B, and C received intraperitoneal injections of recombinant IL-27 (BioLegend, San Diego, CA; 500 ng every three days), an anti-IL-27p28 antibody (anti-IL-27p28-Ab; eBioscience, San Diego, CA; 50 μ g every three days), or phosphate-buffered saline (PBS) (0.1 mL every three days) for 8 weeks, while groups D, E, and F started treatment after 8 weeks of Western-type diet consumption and were treated for 8 weeks.

Techniques: Recombinant, Staining, Western Blot